

The player controls Captain Viridian, who at the outset of VVVVVV must evacuate the spaceship along with the captain's crew, when the ship becomes affected by "dimensional interference". Due to its high level of difficulty, the game world contains many checkpoints, to which the player's character is reset upon death.

These are situated inside a large open world for the player to explore, spanning more than 400 individual rooms. VVVVVV contains eight main levels, including an intro level, four levels which can be accessed in a non-linear sequence, two intermission levels, and one final level, only seen outside Dimension VVVVVV (in a "polar dimension"). Later areas introduce new mechanics such as moving floors or rooms which, upon touching one edge of the screen, cause the player character to appear on the other side. The player uses this mechanic to traverse the game's environment and avoid various hazards, including stationary spikes and moving enemies. This feature was first seen in the 1986 8-bit game Terminus. Unlike most platforming games, in VVVVVV the player is not able to jump, but instead can reverse the direction of gravity when standing on a surface, causing Captain Viridian to fall either upwards or downwards. The "C" icon to the left of the spike pit is a checkpoint, which the player is returned to upon dying. In this room, Captain Viridian must avoid the red spikes and green ghost.
